1. Retention time (tR) is a measure of how long a compound is retained on a...

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Chemistry

1. Retention time (tR) is a measure of how long a compound isretained on a stationaryphase in column in column chromatography.In TLC, Rf was a measure of how long thecompound was retained onthe stationary phase. What is the relationship between tR andRf?
2. What would be the order of elution if one separated thepigments of spinach on columnchromatography?
3. What if any would you have to change in terms of the mobilephase if you wanted toactually perform the column chromatography inorder to separate the pigments of spinachcompare to the one usedfor the TLC Experiment? Explain.
4. Why is it important to keep the solvent level above thestationary phase? What wouldhappen if one lets the solvent get muchlower than the alumina stationary phase?

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Answer 1 Retardation factor Rf is the fraction of an analyte in the mobile phase of a chromatographic paper or sheet It is defined as the ratio of distance traveled by the center of an analyte spot to the distance traveled by the solvent front RF are same as R values in column chromatography Basically in column chromatography the retention factor k is the ratio of time an analyte is retained in the stationary phase to the time it is retained in the mobile phase Retention factor is inversely proportional to the retardation factor If retention factor is k See Answer

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