1. Please describe negative control in terms of transcriptional regulation of gene expression? 2. Please explain the...

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Biology

1. Please describe negative control in terms of transcriptionalregulation of gene expression?
2. Please explain the regulation of Trp operon when Trp-tRNA isavailable in the cell?
3. What are the classes of Transcription factors based on theirregulatory responsibilities? Please explain if this distinction isabsolute or not?
4. Please explain the mitochondrial protein synthesis by indicatingthe mitochondrial protein synthesis apparatus, the mitochondrialgenetic code and so on?
5. Please explain the two hybrid assay?
5. Please explain the two hybrid assay?

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Ans1NegativecontrolThe lac operonis under both negative and positive control The mechanisms forthese will be considered separately1 In negative controlthe lacZYAgenes are switched off by repressor when theinducer is absent signalling an absence of lactose When therepressor tetramer is bound to o lacZYAis nottranscribed and hence not expressedRepressed lacoperon2 When inducer ispresent signalling the presence of lactose it binds therepressor protein thereby altering its conformation See Answer

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