1. Lab Review:
A) Explain why– control E. coli cells cannot grow onLBA+amp and LBA+amp+X-Gal.
B) Name a procedure which can replace the heat shock process andachieve the same goal:
C) If you used the pBLU plasmid as a vector to carry adesirable human gene into E. coli recipientcells. The insertion site is inside the β-galactosidase gene on thep-BLU plasmid. After the transformation, you plate the cells onLBA+amp+X-Gal to screen for E. coli cells with the desirable gene.You saw both blue and white colonies on the plate. Which type ofcolonies carry the desirable human gene?
A. White       B.Blue            C. both           D. neither one
Explain WHY?
