1. In E. coli cell is grown in the presence ofhigh amounts of glucose. Which of the following istrue?

a. The cell will use lactose as a carbon source.

b. The level of cyclic AMP in the cell will be low.

c. The level of cyclic AMP in the cell will be high.

d. Transcription of mRNA from the lac operon will behigh

e. The cell will die.

2. Enhancers are

a. proteins located adjacent to promoters

b. distant nucleotide sequences where gene regulatory proteinsbind

c. nucleotide sequences that block replication and inhibittranscription

d. proteins that bind and deactivate repressors

e. bacterial forms of promoters

3. When tryptophan is present in the media, thetryptophan molecules regulating the trpoperon binds the

a. trpE enzyme

b. trp promoter

c. trp operator

d. trp repressor

e. trp polymerase

4. Your liver and heart cells are differentbecause

a. they contain different genes

b. they contain different operons

c. each contains unique promoters

d. different regulatory genes are expressed in differenttissues

e. they contain different chromosomes

5. In prokaryotic cells, a group of genes that code forfunctionally related and coordinately regulated proteins isa(n)

a. exon

b. intron

c. operon

d. repressor

e. homopolymer

6. Heterochromatin corresponds to densely packed regionsof chromosomes. Hence heterochromatin likely contains:

a. Methylated DNA and hypoacetylated nucleosomes.

b. Methylated DNA and hyperacetlyated nucleosomes.

c. Unmethylated DNA and hyperacetylated nucleosomes.

d. Unmethylated DNA and hypoacetylated nucleosomes.

e. Degraded DNA and degraded nucleosomes.

7. The I^s mutation yields asuper repressor of the lac operon. Themost likely cause of this phenotype is:

a. The operator region no longer binds I^srepressor.

b. The promoter element shows irreversible binding to theinducer, lactose.

c. The beta-galactosidase gene is active.

d. The I^s repressor protein can no longer bind theinducer lactose.

e. The I^s repressor binds ribosomes, blockingexpression of the lac operon.

8. Attenuation is aform of transcription regulation

a. that can be manifested by coupling transcription andtranslation .

b. that permits the photolyase reaction to repair DNA duringtranscription.

c. that results in degradation of amino acids not essential togrowth.

d. that yields progressive increases in disease severity withage.

e. that can only be manifested through trans-activation ofadjacent operons.

9. Multi-cistronic (polycistronic) messengerRNAs

a. Are only found in eukaryotic cells and encode genes for thecistronic organelle.

b. Encode transfer RNAs found inside bacterial mitochondria.

c. Distinguish between splice variants during the constitutivegrowth phase.

d. Is a single messenger RNA encoding multiple, different geneproducts.

e. Encode a single polypeptide chain.

10. An insulator functions to

a. Insulate ribosomes from enhancer effects

b. Insulate mRNAs from exonuclease activity

c. Insulate cells from the effects of bacterial endotoxins

d. Insulate specific introns from splicing.

e. Insulate genes from the effects of an enhancer