1. In CRISPR, why does it make sense to design our crRNA first before making any...

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Biology

1. In CRISPR, why does it make sense to design our crRNA firstbefore making any other changes to the genomic DNA?

2. In CRISPR, when searching for PAMs, GGNGG makes a much betterPAM than NGG - why is this?

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CRISPERcas9 is a method of gene editing which uses cas9 enzyme which used as scissors to cut at a specific region of double bond genomic DNA whereas a small predesigned RNA sequence called guideRNA or    See Answer
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