1) If you suspect your culture of bacteria has 125 x 106 cells per mL, what...

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Biology

1) If you suspect your culture of bacteria has 125 x 106 cellsper mL, what would you want the final dilution to be in order toend up with 50 cfu on a plate?
2) A 0.1 dilution is performed on a culture of bacteria in orderto perform viable plate counts. From the dilution,0.1 mL ofsolution is plated on solid media, and 154 colony forming unitsgrow on the plate. How many bacteria are in a single mL of theoriginal culture?
3) How many microliters of original sample arerequired to produce a final dilution of 10^-3 in a totalvolume of 5 mL? 1 microliter is 10^-6 L or10^-3 mL.
4) There are so many microbes in a single mL of culture, it isvery difficult to perform one dilution to produce countable cells.Microbiologists need to perform a dilution series, where multipledilutions are performed in sequence to arrive at the correctdilution.
Dilutions are cumulative. Multiply the series of dilutionstogether to find the final dilution value.
If 6 serial dilutions are performed, each with a dilution of0.01, what is the cumulative dilution?

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Answer 1 If you suspect your culture of bacteria has 125 x 106 cells per mL what would you want the final dilution to be in order to end up with 50 cfu on a plate Number of bacteria in culture 125 x 106 cells per mL Required number of CFU on a plate 50 If the volume of culture used on plate is example 100ul then dilution factor 250000 25 x 105 If the volume of culture used on plate is example 1ml or 1000ul then Fold See Answer

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