1. If the enzyme-catalyzed reaction E + S ↔ ES ↔ E + P takes place...

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Chemistry

1. If the enzyme-catalyzed reaction E + S ↔ ES ↔ E + P takesplace near Vmax to the enzyme (E), what can be concluded about therelative concentrations of S and ES?

a) High [S], [ES] is low

b) High [S], [ES] are at their maximum

c) Low [S], [ES] is low

d) Low [S], [ES] is at its maximum

2. Which of the following statements about Michaelis-Mentenkinetics is correct?

a) Km = the substrate concentration required for the reaction toreach Vmax

b) Km = dissociation constant of the enzyme-substratecomplex

c) Km = is a measure of the substrate's affinity for theenzyme

d) Km = expression of reaction rate

3. What is the Km value if the substrate concentration is 25 mMand the initial reaction rate is half Vmax for an enzyme catalyzedreaction?

a) Km = 50 mM

b) Km = 12.5 mM

c) Km = 625 mM

d) Km = 25 mM

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