1. How does an automated gene sequencer detect the
specific nucleotide at the end of a...
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Biology
1. How does an automated gene sequencer detect thespecific nucleotide at the end of a DNA fragment?
2.How is genotyping different from genomesequencing?
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Ans 1 Automated DNA sequencing utilizes a fluorescent dye to label the nucleotides instead of a radioactive isotope The fluorescent dye is not an environmentally hazardous chemical and has no special handling or disposal requirements Instead of using Xray film to read the sequence a laser is used to stimulate the fluorescent dye The fluorescent emissions are collected on a charge coupled device that is able to determine he wavelength The PerkinElmer Applied Biosystems ABI DNA
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