1. Describe the requirements and caveats to culturing animal cells; what nutrients and materials/substrate are required;...

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Biology

1. Describe the requirements and caveats to culturing animalcells; what nutrients and materials/substrate are required; somecell types need special growth substrates (behave differently insuspension â€“ unlike in tissue)
2.Explain a primary culture vs. a cell line and the strikingdifference that tends to permit cell lines to be isolated
3. Understand how chemical screens are utilized

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ANSWER TO 1 Animal cells unlike microbial culture requires a variety of nutritional factors like amino acids vitamins hormones growth factors Microbes can synthesize several amino acids on their own but animal cells cant Hence it must be supplemented with the media To fulfill this we add serum to the media egFBS Foetal Bovine Serum which contains several of the abovelisted substances Generally antibiotics are also added to prevent microbial contamination a CO2 Bicarbonate based buffer like HEPES is also used to ensure proper pH The temperature is 37 degree celsius Oxygen pressure See Answer

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