Logo
Home Study Material Contact Search
AI Chat FREE
  1. sciences
  2. biology
  3. 1. describe the mechanism of dcfda and, what is th

1. Describe the mechanism of DCFDA and, what is the purpose of running assay 2 How will...

60.1K

Verified Solution

Question

Biology

1. Describe the mechanism of DCFDA and, what is the purpose ofrunning assay

  1. 2 How will we evaluate the cytotoxicity of the 3 compounds weare working with?Must describe the mechanism of CCK-8.

Answer & Explanation Solved by verified expert
4.1 Ratings (732 Votes)
DCFDA Cellular ROS levels can be measured in live cells by a technique that converts 27 dichlorofluorescin diacetate DCFDA which is oxidized to a fluorescence dye 2 7 dichlorofluorescein DCF The fluorescence generated is directly proportional to the amount of oxidized DCFDA to DCF Reactive oxygen species ROS such as superoxide and hydrogen peroxide are continually produced during metabolic processes ROS generation is normally    See Answer
Get Answers to Unlimited Questions

Join us to gain access to millions of questions and expert answers. Enjoy exclusive benefits tailored just for you!

Membership Benefits:
  • Unlimited Question Access with detailed Answers
  • Zin AI - 3 Million Words
  • 10 Dall-E 3 Images
  • 20 Plot Generations
  • Conversation with Dialogue Memory
  • No Ads, Ever!
  • Access to Our Best AI Platform: Flex AI - Your personal assistant for all your inquiries!
Become a Member

Other questions asked by students

Chemistry
1.2K views

Using the standard values of enthalpy changes (ΔH°) and entropy change (ΔS°) to calculate the Gibbs...

Medical Sciences
1.2K views

Explain how flow through a blood vessel is determined. Explain what a “pressure difference” is and...

Psychology
1.2K views

Complete the X-Y Questionnaire located at the end of chapter 1 in Organization Behavior in Health...

Mechanical Engineering
1.2K views

What are the similarities and differences with AutoCAD and Solid Works? Any pros or cons to using...

Basic Math
1.2K views

Make a Frequency Distribution Chart for the following set of Data 50, 10, 25, 20, 20,...

Accounting
1.2K views

Cane Company manufactures two products called Alpha and Beta that sell for $120 and $80, respectively....

Physics
1.2K views

41 The terminal potential difference across A and B in the circuit shown is 1...

Accounting
1.2K views

John Fleming, chief administrator for Valley View Hospital, is concerned about the costs for tests...

Accounting
1.2K views

3. At January 1, 2017 the Leon White Company had 400,000 shares of common stock...

Accounting
1.2K views

Tombro Industries is in the process of automating one of its plants and developing a...

Accounting
1.2K views

Corp is a manufacturer and has beginning inventory of 210 finished units, expected sales of...

Accounting
1.2K views

Daniel acquires a 30% interest in PPZ Partnership from Paolo, an existing partner, for $39,000...