1) describe isoelectric focusing and isopycnic (density) separation techniques 2) why are these real focusing techniques?...

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Chemistry

1) describe isoelectric focusing and isopycnic (density)separation techniques 2) why are these real focusing techniques? 3)how are these gradients established in both cases? d) describe thedifficulties in using each 4) why is chromatofocusing not a realfocusing technique? 5) describe advantages of these techniques andgive examples

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Isoelectric focusing IEF also known as electrofocusing is a technique for separating different molecules by differences in their isoelectric point pI It is a type of zone electrophoresis usually performed on proteins in a gel that takes advantage of the fact that overall charge on the molecule of interest is a function of the pH of its surroundings Isopycnic means of the same density In particular an isopycnic surface is a surface of constant density This term is a bit more obscure than the similar terms isobaric or isothermal surfaces which describe surfaces of constant pressure and See Answer

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