1. Can iodine be added before the primary stain in a Gram stain? Explain. If iodine...

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Biology

1. Can iodine be added before the primary stain in a Gram stain?Explain. If iodine was added before the primary stain, what wouldbe the final color of gram positive and gram negative cells in thestain and why?
2. Since you canâ€™t identify bacteria from a Gram stain, whymight a physician perform a Gram stain on a sample beforeprescribing an antibiotic?
3. The cell envelope of Mycobacterium sp. is unlike the envelopeof other bacteria. While genetic evidence indicates that theseorganisms are related to gram-positive bacteria, one could arguethat their structural characteristics could place them in placethem among the gram-positives or the gram-negatives. Explain.

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3.6 Ratings (305 Votes)

1 Iodine is used as a mordant in Gram staining Mordant means trapping agent It is used for trapping the Crystal voilet molecules to make big sized Crystal Violet Iodine molecules These molecules struck into the peptidoglycan meshwork and impart purple colour to Gram Positive Bacteria So even if we add iodine before Crystal Violet stain then See Answer

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