1. Calculate how to make 500.0 mL of 0.100 M triethanolaime solution. 2. You need to prepare...

60.1K

Verified Solution

Question

Chemistry

1. Calculate how to make 500.0 mL of 0.100 M triethanolaimesolution.
2. You need to prepare a buffer for biochemistry lab. Therequired solution is 0.5 M sodium phosphate, pH 7.0. Use theHenderson-Hasselbach equation to calculate the number of moles andgrams of monobasic sodium phosphate (NaH₂PO₄)and dibasic sodium phosphate (Na₂HPO₄)necessary to make 1 liter of the solution.
3. Dscribe how you would prepare a 0.1 M glycine buffer of pH10.0. You have available isoelectric glycine and sodium glycinate.Does it matter if you use sodium glycinate or potassium glycinatefor buffer? Why or why not?
4. Describe how you would prepare a 0.20 M Tris-HCl buffer of pH8.0. The only Tris reagent you have available is Tris base. Whatother reagent do you need, and how would you use it to prepare thesolution?
Please show all work. I appreciate the help!

Answer & Explanation Solved by verified expert

4.4 Ratings (815 Votes)

CALCULATION OF NUMBER OF MOLES OF Na2HPO4 ANDNa2HPO4 Na2HPO4 15135 NaH2PO4This means that if the concentration of NaH2PO4 is 1 mol L thethe concentration of Na2HPO4 is15135 mol LHere the See Answer

Get Answers to Unlimited Questions

Join us to gain access to millions of questions and expert answers. Enjoy exclusive benefits tailored just for you!

Membership Benefits:

Unlimited Question Access with detailed Answers
Zin AI - 3 Million Words
10 Dall-E 3 Images
20 Plot Generations
Conversation with Dialogue Memory
No Ads, Ever!
Access to Our Best AI Platform: Flex AI - Your personal assistant for all your inquiries!

Become a Member