1) After taking an aliquot of your cells and diluting them with Trypan Blue in a...

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Biology

1) After taking an aliquot of your cells and diluting them withTrypan Blue in a 1:1 dilution, you load the cell/Trypan mixtureonto a hemocytometer to count the cells.
You obtain the following counts:
Quadrant Transparent cells Blue cells
1 65 2
2 71 4
3 68 1
4 73 3
5 67 2
What is the average number of Trypan-diluted cells perquadrant?
2) Now, take into consideration the Trypan Blue dilution. Whatis the more concentrated number of cells per quadrant?
3) What is the volume in each quadrant of a hemocytometer?
4) What is the cell density (concentration) ofthe cells (cells/mL)?
5) If your original cell culture (the one you took an aliquotfrom to count) has 13 mL of cell suspension, what is thetotal number of cells you have in culture?

Answer & Explanation Solved by verified expert

3.9 Ratings (576 Votes)

1 Trypan blue dye is used in the dye exclusion test to determine the viable cells in a given culture The viable or intact cells exclude or do not take up the dye and apeear to be transparent cells while the dead cells take up the dye and appear in blue colour Average no of cells per See Answer

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