1) After taking an aliquot of your cells and diluting them with Trypan Blue in a...

90.2K

Verified Solution

Question

Biology

1) After taking an aliquot of your cells and diluting them withTrypan Blue in a 1:1 dilution, you load the cell/Trypan mixtureonto a hemocytometer to count the cells.
You obtain the following counts:
Quadrant Transparent cells Blue cells
1 65 2
2 71 4
3 68 1
4 73 3
5 67 2
What is the average number of Trypan-diluted cells perquadrant?
2) Now, take into consideration the Trypan Blue dilution. Whatis the more concentrated number of cells per quadrant?
3) What is the volume in each quadrant of a hemocytometer?
4) What is the cell density (concentration) ofthe cells (cells/mL)?
5) If your original cell culture (the one you took an aliquotfrom to count) has 13 mL of cell suspension, what is thetotal number of cells you have in culture?

Answer & Explanation Solved by verified expert

3.9 Ratings (576 Votes)

1 Trypan blue dye is used in the dye exclusion test to determine the viable cells in a given culture The viable or intact cells exclude or do not take up the dye and apeear to be transparent cells while the dead cells take up the dye and appear in blue colour Average no of cells per See Answer

Get Answers to Unlimited Questions

Join us to gain access to millions of questions and expert answers. Enjoy exclusive benefits tailored just for you!

Membership Benefits:

Unlimited Question Access with detailed Answers
Zin AI - 3 Million Words
10 Dall-E 3 Images
20 Plot Generations
Conversation with Dialogue Memory
No Ads, Ever!
Access to Our Best AI Platform: Zin AI - Your personal assistant for all your inquiries!

Become a Member

1) After taking an aliquot of your cells and diluting them with Trypan Blue in a...

90.2K

Verified Solution

Question

Biology

Answer & Explanation Solved by verified expert

Get Answers to Unlimited Questions

Membership Benefits:

Other questions asked by students

You suspect that a railroad embankment is unstable and exhibiting a slip failure. To confirm this...

A 4.0-cm-diameter parallel-plate capacitor with a 1.0 mm spacing is charged to 1000 V. A switch...

Heat is useful in the destruction of bacteria because heat...denatures enzymes by altering their 3D...

0 A plane electromagnetic wave E 100 sin 5 x 108 t 3x V m...

7 Two masses m 3 60 kg and m 5 75 kg are connected by...

OS 7 Area and perimeter in the coordinate plane II MHQ Graph quadrilateral FGHI with...

What s the relationship between systems and matrices How do you a matrix to solve...

If 0 7 then 4 sin 0 equals cos 0 equals tan 0 equals sec...

Define automatic reinstatement.

Exercise 11-09 b (Essay) On January 1, Graves Corporation had 60,000 shares of no-par common...

Verify that the mix of sales units to be sold at the break-even point for...

If my risk of material misstatement is high, I need to set my ___________ at...