1) A bacterial strain called XYZ living in the lake MBG223 nearBilkent University requires to synthesize tryptophan continuously.The reason is, the essential proteins (essential means, theproteins required for survival of the bacteria) contain highcontent of tryptophan. Therefore, this bacterial strain XYZ has atryptophan operon. Unfortunately, the tryptophan level of nutritionavailable in lake MBG223 is very limited during summer and wintertime and the other nutrition levels are high. During the summertime, as the lake MBG223 partially evaporates, the salinity of thewater increases, the temperature of the lake arises and the pH ofthe environment turns from natural to acidic. Changes in salinity,pH and temperature is known to disrupt hydrogen bonding innucleotide base-pairings and also the structure of the proteins.Here, assuming that the structure/function of the proteins are noteffected please answer the following.
Ä°nside the XYZ bacteria, what would you observe with regards tosynthesis of tryptophan bearing proteins during summer vs. wintertime? How would this effect bacterial growth rate during the summertime vs winter time?
2)In another neighboring lake called MBG222, they found adifferent bacterial strain called WOWWW to have mutant tRNAs on theanti-codon region of the tRNA. This tRNA carries Phenylalanine towherever it encounters the sequence UGG (tryptophan) on the mRNA.What happens to the regulation of the trypthophan operon inbacteria that has 1) high tryptophan and low phenylalanine vs.bacteria that has low tryptophan and high phenylalanine?
